Blogs

The temperature profile shows a drop in ocean temperature to about 7 degrees C, just off the coast of Washington near Grays Harbor.
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The oxygen profile show a decrease in dissolved oxygen.
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Chlorophyll profile
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My last few blog entries probably seem a bit cryptic, but it's been hard to figure out what exactly to put down. Going back a few steps-one of our original hypotheses was that the FNR-1 binding site of the anthracis narG promoter was responsible for aerobic nitrate reductase activity in anthrax.  We isolated six strains of B. subtilis carrying the anthracis narG promoter (two with both binding sites intact, two with one site deleted, two with both sites deleted).To determine whether or not this promoter was active, we used a lac assay.

Week 7 and I am still loving this job. As I mentioned last week we were able to move on to hybridizing Pseudo-nitzchia samples and we were very successful. Each chip worked this time after some carefull cleaning.   The reliability of the chips is proving to lessen after about two uses but luckily we have enough potential uses to get all the hybridiztions done.  To end the week off I mostly labeled PCR products with Biotin...that was about 36 total which should conclude all samples need to be prepared for hybridization for my ten week period.

today is friday the last day of week four. this happens to be the only day i was here this week and its pretty laid back today. there were some visitors here today who came to learn about cmop, i sat in on the presentation and on the pizza lunch lol. also monica and matt gave me two presentations that they put together. it was pretty impressing.

This week, I worked on trying to solve The Great Mystery of the Zn Fluorescence! What ghost is hiding in the Fluoromax and making my zinc values way too high? Too bad Scooby Doo isn't around to help me with this one.

This week I’ve accomplished a lot more compared to the previous weeks, and there is yet more to come. Now that I got the line, this week I focused on putting a model to follow that line. I first started this by making a placemark to follow the line. After the placemark, I decided to place a 3-d model. However, this came with its price, because unlike a 2-d placemark, a 3-d figure has many other variables that one has to keep in mind.

This week has been busy. Nate gave us all of the Phoebe data and showed us how to compile it all into one place in MATLAB. After doing that, we were able to graph all the Phoebe data at once. After Nate talked with Joe Cho, they decided that it would be helpful to see the data in horizontal layers. Again, Nate showed us how to manipulate the data so that we could use data that was only taken from a specific depth, 0 to 2.5 meters. With this, we were able to compare this layer's salinity with the model's prediction of what the layer's salinity would be.

A fairly uneventful week... I have been running the purifications on my own and busting out as much PHM as I can (which in the end turns out to be a discouragingly small quantity) But I have gotten very comfortable with the procedure and can now multitask two of the steps at once!

Wow, time has flown! A fairly uneventful week... I have mostly just been getting comfortable with the purification process on my own. There are a lot of steps to remember, but with practice I think I will get the hang of it.

Currently I am running a batch of PHM which was produced at the very end and so there is very little in it. For example I'll start with 1L and in the end get about 1mL! This is different then what I was working with before as I was getting a bit more out of the purification. Oh well!