Blogs

At the beginning of this week I finished up amplifying my samples and started the DGGE process.

On one hand it's been more of the same - the experiment is just a simple algorithm and I go into the lab, perform it, and that's that. On the other we got a hold of some info on a conference called SACNAS that will take place at Dallas in October. We submitted an abstract and will hear back from them within a few weeks. I just have to put together this final report and presentation and that'll be that for now.

On one hand it's been more of the same - the experiment is just a simple algorithm and I go into the lab, perform it, and that's that. On the other we got a hold of some info on a conference called SACNAS that will take place at Dallas in October. We submitted an abstract and will hear back from them within a few weeks. I just have to put together this final report and presentation and that'll be that for now.

So this week I discussed with my mentors the final pieces to my project, of what could and could not be done by the time I’m out. And I also got useful suggestions of how to calculate the speeds for the glider’s movement and also the angle tilt. One of the biggest mistakes I did in my calculations was omitting the depth.  Once again, for some reason I was thinking 2-dimensional. However, now I am glad to have figured out the problem.  Also, something that was ruining this week was my molar tooth ache.

These weeks seem to go by faster and faster. I can't believe that I will be done with my project in one week from now. I also am very nervous about finishing everything on time.

We haven't finished writing the density program for Matlab, so I will have to spend some time on that next week.

We also revamped the density equation that we are using and found some of the basic assumptions that we need to make to get a one to one relationship between floc density and floc Diameter.

So I can't handle how fast this week went by!  It was pretty jam packed....  lots of different PCR reactions, gel purification, cloning galore and sampling!

The cryptophyte rubisco primers are working really well and next week we will have sequences for multiple sample locations and dates! We will have sequences for peak red tide blooms for 2007 and 2008, Ilwaco on June 30th, Ilwaco on July 21st, Willapa on June 30th and Willapa on June 23rd (different sampling team). I am very excited to get all the sequences back and compare them!

I can't believe that week 8 has already come and gone. This week was really the most indicative of my readiness/willingness to go straight on to Grad school. I know that at grad school, you have to be much more of a self-directed learner and I am not really ready for that yet. I do hope to go on to grad school, but at a time where feeling completely lost every day does not seem so daunting.

Not a whole lot going on now, I've finished all of my plasmid constructs and have transformed them all into B. subtilis. I'll be doing one final lac assay Monday-Tuesday for the narG promoter containing a nucleotide insertion-I won't have time to finish everything and I really want to see if this one turns out to be correct.

Whoa this week went by tooo fast. This week I found out that the MatLab JA Builder does not allow you to deploy GUIs in a web application so now I will convert my GUI into a standalone application using the MatLab Compiler. I also implemented another feature into my application: a way to keep track of the flags you set by saving the image to the user's computer. After this my GUI was looking pretty good and it was on to testing it out....the scariest part!

The centers best and brightest take a break for a game of soccer.

Soccer Match - Nirzwan and Chelsea square off