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Week 4: Midterms & Mapping Out More Model Data

Monday and Tuesday: The week started with beginning each of the suggested tasks from my presentation last Friday. I’ve begun the process of using Forecasts 22, 26, and 28 for SATURN-07 in May so that I may make a comparison of the different model data values and see if there is an increase or decrease in error.

Week ?

 After meeting with one of my future users (there aren't very many of them) I've been given a few more things to add to my interface. However, a ten day break to go to Italy has not given me much time to actually add the new features. The only real change I've made since my last blog was to add little calender symbols by the start and end time shufflers (what I've taken to calling a series of buttons that allow a user to adjust the start and end time of the graph).

Week Six: Midterms, hiking, and extractions, oh my!

What a busy week.  We're almost done working directly with our samples, and will be doing lots of data analysis in the upcoming weeks.

On Monday, we worked on purifying our day 0 RNA.  The RNA extraction procedure doesn't distinguish between RNA and DNA and so we need to remove the DNA contamination from our RNA samples. 

Running New Samples through the uFCM! (Week 4)

This week I continued working with LabVIEW programming. I added more peak detectors with different threshold values. This way, I can program it to subtract between the different thresholds to get a more accurate count of the number of particles. As the program becomes more sophisticated, it will essentially replace the need to do any analyzing in excel. I also got the chance to run new samples through the uFCM. The 5 um and 20 um beads arrived and I made concentrations with 10 drops of bead stock in 40 ml of deionized water for each bead size.

Is it Local or Regional, and Midterms Already!! (Week 6)

This was a pretty straight forward week!! It simply began and ended. I spent most of my time finalizing my coding scripts to get them ready for my model run that will be finished on Monday! I can not wait to get the run and start extracting data from it and conducting my comparisons. I also can not wait to conduct an analysis of Physical Habitat Opportunity for Salmon. I did, however, find what I think is a potential error with in the Forecast-26 Model.

A Taste of Graduate School, Testing pH Effects, and a Mount Hood Adventure

What a week!  There was never a dull moment this week.  I got my first taste of a graduate school experience on Monday when my Principal Investigator asked me to prepare a presentation of the work that I have done over the past few weeks and present it in front of him and another research group.  It wasn't a formal presentation but rather a casual presentation meant to inform everyone of my progress and create an environment where ideas about how to move forward could be discussed.  It was a great experience and took away a lot of anxiety about presenting in front of res

Week 4 - Phylogenies and presentations

After having identified an abundance of coxL in the Warren cave metagenome last week, I spent the first half of this week building a phylogenetic tree in order to learn more about the evolutionary radiation of the coxL gene and the relatedness of the cave samples to cultured and uncultured microbial species. In order to begin the tree-building process, I first had to align the contiguous sequences from the metagenome with the coxL IMG database and translate the nucleotide sequences to amino acids.

Week 3: I’ve got no strings

       This week I felt a lot more confident in threw me off a little bit, due to the holiday in the middle of the week. Before this point, I never thought I would confuse Wednesday with Sunday. But nonetheless, the work continued and the day off gave the bacteria an extra day to grow.

Begining to infect my B.subtilis & some frustrations (week 4)

This week has been a mixed bag.  It started out well.  I got to begin by learning how to transform the Bacillus subtilis and by inserting my whole promoter sequence (which came back successfully two weeks ago from sequencing) into the wild type strain of competent cells I generated last week.  This involved making DSM agar with Erythromycin as the antibiotic resistance selection agent (since the plasmid will give this resistance to the

Week 4 Mid-term presentation week

             This week turned out to be a relative slow week, compared to my last few weeks at CMOP. Last week, Ben and I prepared Iwaco Harbor and main channel station # 7 prebloom/bloom/postbloom dinoflagellate 18S rDNA samples, and transformed the E.

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