This week we read a few jounal articles that had to do with finding where the NsrR binds and how this site would be affected if you have site-directed mutagenesis. At the moment i am still learning and researching the different genes that are controlled by NsrR during anaerobic respiration. Also, the professor went over various concentration concepts with dilutions and molarity.
With my first week of work at CMOP coming to an end I can honestly say I have been enjoying myself. Working with my mentor Jon Schnorr I have been able to complete two Polymerase Chain Reaction (PCR) amplifications of Archaea DNA samples collected from the Columbia River plume by Dr. Mariya Smit. I believe the goal of this project is to study the microorganisms of the Columbia River, its estuary, and plume using the PCR method of DNA analysis.
This week, I met all the interns, CMOP staff, and the people that'll be working in the same lab as me (Dr.Needoba's, aka Joe's lab). I am slowly beginning to understand what my project for the summer will entail. I've read a lot of background information; the idea of using fluorescence to measure the amount of ammonia in a sample is wholly new to me. I also conjured up some ammonia standards of varying concentrations, and I made a working reagent solution, which I need to mix with the standards before running them through the fluorometer.
The CMOP field team set out on their second glider deployment. The seas were calm as we traveled about 25 miles out into the ocean to launch the glider.
Danny helps deploy the glider off the ship.
The gliders name is Phoebe. The rope on her tail end is only there during system tests.
We deployed Phoebe today and sent on her way towards the Gray's Harbor line. The mission will send her on a zigzag line from north of Gray's Harbor back down past the Columbia River entrance.
This week, Andrew was able to locate some B.neritina ovicell samples, so now I am well on my way with my project! It is my job to locate ovicells that are "full," do FISH on those samples, and localize E.sertula. By looking at ovicells at different developmental stages, we will be able to better understand E.sertula's movement within the ovicell prior to the release of the larvae. It is important to note that much work has been done, especially by Dr. Haygood and associated researchers, on the location of E.sertula during the larval stages and beyond.
I am happy to say I am back for a second summer internship at CMOP! I am working in the same lab, Dr. Haygood's, and with the same mentor, Andrew Han. Although I am working with the same people and in the same environment, I have an entirely new and unique project to tackle!
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