Week 8

7-27
-Inoculated all 7 mutant strains (E1, E2, E3, C1, C3, F1, D1) for 5 hours starting at 10:21am then plated onto YE% screening plates.
-Made LBKan (with proper amount of Kan - I used 6µl for the previous plates for some reason instead of 600µl) and YE% plates again. YE 225µl did not mix terribly well. It appeared to solidify too quickly.
-Repeat transformation (plated onto wrong amount of LBKan plates, so this will be redone tomorrow)
- O/N cultures for two transformants which did grow correctly (F1, D1).

7-28
The mutants F1 and E1 both were at acceptable levels of concentration (ng/µl) as well as appearing to have a quality plasmid based on a 1% gel  separation ran for ~85 minutes
YE % plate pictures ~24hrs
0µl

25µl

50µl

75µl

100µl

125µl

150µl

175µl

200µl

I repeated the transformation and plated onto LBKan with 600µl added (the correct amount). All of the strains grew colonies, and I suspended 3 colonies from the 4 strains I have yet to characterize to grow for overnight culture.

7-30
I mini-prepped the transformants according to the previous procedure, and then set up the digest for the 1%gel run. I also collected microdot data which appeared good.