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Week 3

6-22
Of the mutants plated last week, B1, B2, B3, C1, C2, C3, C4, D1, D4, E1, E2, E3, F1, G3, H1, and H2 were determined to be IMO and thus now are going to be screened and characterized. The screening will begin as follows:

Mutant Lept LeptKan LBMn Motility
B1        
B2        
B3        
C1        
C2        
C3        
C4        
D1        
D4        
E1        
E2        
E3        
F1        
G3        
H1        
H2        

The screening will occur with "x" 1-50 plates with WT plated onto 1 and 2 with the rest of the key as follows:

Plate 1: C1 - 7, 9, 11, 12 / C2 - 13, 15, 17, 19 / C3 - 29, 31, 33, 35 / C4 37, 39, 41, 43
Plate 2: B1 - 13, 15, 17, 19 / B2 - 29, 31, 33, 35 / B3 - 37, 39, 41, 43
Plate 3: D1 - 13, 15, 17, 19 / D4 - 29, 31, 33, 35 / F1 - 37, 39, 41, 43
Plate 4: E1 - 13, 15, 16, 19 / E2 - 29, 31, 33, 35 / E3 - 37, 39, 41, 43
Plate 5: H1 - 13, 15, 17, 19 / H2 - 29, 31, 33, 35 / G3 - 37, 39, 41, 43