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Week 5
Week 5 was pretty busy. We did two lac assays-the first seemed to support our hypothesis, but there were some strange results on the second, so we're taking a few steps back this week and looking at our Bacillus strains. I also used two-step PCR to introduce a mutation into the anthracis narG promoter. If everything goes as planned, we should be able to sequence the (hopefully) mutated promoter and continue from there.