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Week 2- Laboratory work
Submitted by Vena Haynes on June 18, 2010 - 3:28pm
This week I continued to do PCR of our target dsDNA. Two of our genes had clear enough bands and were the right length to continue on with purification, and so I purified our PCR samples and determined the DNA concentration.
Because we are waiting on an order of new primers, I began RNA extraction of ocean water samples. The first time I assisted in the extraction, and the next two times I performed the extraction on my own. The next step with our RNA pellet is to completely purify by removing any DNA present with DNase.
Next week I will be both here and in Vancouver at Sharp Lab of America running samples on the new sensor arrays.
-Vena
