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Week 8
7/29
So far this week I am finishing up hybridizing the rest of the selected PCR products. Some of the chips are still hard to work with and require very careful handling and cleaning. The chips aren't guaranteed to, but they should work for about 4 uses. So far two out of 8 chips are not working after about 2-3 uses. However, we have enough to finish up the rest of the hybridizations this week and start analyzing the results.
7/31
We actually managed to get clear readings from all hybridized samples this week which included 24 total! There were quite a few meetings this week so next Monday and Tuesday I will finish up the last 12 samples to be labeled and hybridized. Then, the only step is interpreting and analyzing the results for not only my paper but the main research paper for Mariya and Holly as well. I have already began writing my own paper required for the internship and so far it is going well. Mariya is gone next week so I will finish the rough draft before she gets back.
Pictured below is a diagram of a fully labled and hybrized oligonucleotide probe and how energy tranfser will give you clear readings from ElectraSense.